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Anion exchange polymeric sorbent coupled to high‐performance liquid chromatography with UV diode array detection for the determination of ten N ‐nitrosamines in meat products: a validated approach
Author(s) -
Iammarino Marco,
Mangiacotti Michele,
Chiaravalle Antonio E.
Publication year - 2020
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.14410
Subject(s) - sorbent , chemistry , chromatography , detection limit , extraction (chemistry) , selectivity , analytical chemistry (journal) , chromatography detector , solid phase extraction , high performance liquid chromatography , adsorption , organic chemistry , catalysis
Summary Among mechanisms and molecules presumably involved in the carcinogenicity induced by meat consumption, the N ‐nitrosamines ( N ‐ NA s) are a class of compounds characteristic of processed meats. In this work, an analytical method for the determination of ten N ‐nitrosamines ( N ‐nitrosomorpholine, N ‐nitrosomethylethylamine, N ‐nitrosopyrrolidine, N ‐ n itrosodiethylamine, N ‐nitrosopiperidine, N ‐nitrosodipropylamine, N ‐nitrosodimethylaniline, N ‐nitrosodibutylamine, N ‐nitrosodiphenylamine and N ‐nitrosodibenzylamine) in fresh meats and meat products was developed, optimised and validated. The method is based on optimised sample purification by solid‐phase extraction (anion exchange polymeric sorbent) and separation/detection by high‐performance liquid chromatography coupled to UV diode array detection. The validation procedure allowed to ascertaining good analytical performances in terms of sensitivity, selectivity towards interfering compounds, accuracy and robustness. The values obtained for precision (range: 4.3–14.4%) and recovery percentages (range: 80.8–95.1%) were compared to reference values indicated in the Decision No. 657/2002/ EC , resulting as compliant. The measurement uncertainty (lower than 14.6%) was satisfactory for each N ‐ NA as well.