Evaluation of pre‐slaughter low‐current/high‐frequency electrical stunning on lipid oxidative stability, antioxidant enzyme activity and gene expression of mitogen‐activated protein kinase/nuclear factor erythroid 2‐related factor 2 (MAPK/Nrf2) signalling pathway in thigh muscle of broilers | Zendy

Xu Lei | Zendy; Wang Jing | Zendy; Yue Hongyuan | Zendy; Farnell Morgan B. | Zendy; Yang Haiming | Zendy; Wang Zhiyue | Zendy; Qi Guanghai | Zendy

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Evaluation of pre‐slaughter low‐current/high‐frequency electrical stunning on lipid oxidative stability, antioxidant enzyme activity and gene expression of mitogen‐activated protein kinase/nuclear factor erythroid 2‐related factor 2 (MAPK/Nrf2) signalling pathway in thigh muscle of broilers

Author(s) -

Xu Lei,

Wang Jing,

Yue Hongyuan,

Farnell Morgan B.,

Yang Haiming,

Wang Zhiyue,

Qi Guanghai

Publication year - 2020

Publication title -

international journal of food science and technology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.831

H-Index - 96

eISSN - 1365-2621

pISSN - 0950-5423

DOI - 10.1111/ijfs.14402

Subject(s) - stunning , antioxidant , malondialdehyde , mapk/erk pathway , oxidative stress , protein kinase a , glutathione , gene expression , endocrinology , medicine , oxidative phosphorylation , kinase , biology , chemistry , enzyme , biochemistry , gene , ischemia

Summary This study was aimed to evaluate the effects of pre‐slaughter low‐current/high‐frequency (LH) electrical stunning (ES) on lipid oxidative stability, antioxidant enzyme activity and gene expression of mitogen‐activated protein kinase/nuclear factor erythroid 2‐related factor 2 (MAPK/Nrf2) signalling pathway in thigh muscle of broilers. Eighteen birds were randomly allocated to the following three treatments with six replicates per treatment and one bird per replicate: without stunning (control); water‐bath ES with sinusoidal alternating LH (86 mA, 1000 Hz, LHES) or high current/low frequency (HL) (130 mA, 60 Hz, HLES). Stunning methods did not affect malondialdehyde level (d 0 ~ d 9) or antioxidant enzymes’ activity at d 0 ~ d 2. LHES enhanced glutathione S‐transferase activity at d 3 and gene expression of MAPKs and Nrf2 compared with HLES. In conclusion, LHES enhanced gene expression of MAPK/Nrf2 pathway, whereas had no superiority over HLES in lipid oxidative stability (4 °C, 9 days) of thigh muscle in broilers.

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