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Immunomodulatory properties of selectively processed prawn protein fractions assessed using human peripheral blood mononuclear cells
Author(s) -
Faisal Md,
Dargahi Narges,
Vasiljevic Todor,
Donkor Osaa.
Publication year - 2020
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.14331
Subject(s) - antigenicity , immunogenicity , chemistry , peripheral blood mononuclear cell , biochemistry , prawn , microbiology and biotechnology , vitellogenin , antigen , biology , immunology , in vitro , gene , fishery
Summary Prawn muscles were treated with acetic acid and high‐pressure processing (600 MP a) separately to analyse their antigenicity and immunogenicity. The protein fractions were separated and isolated using preparative HPLC , and their antigenicity was analysed using Immunoglobulin G (IgG) ELISA kit. Out of thirty‐nine protein fractions, only four (A10, A11, B10 and C9) were detected with antigenic potentials. The immunogenicity of these protein fractions was analysed using human PBMC s, and supernatants were collected at multiple times from 0 to 144 h. The treated fractions (B10 and C9) analysed using Immunoglobulin E (IgE) ELISA kit showed significantly ( P < 0.05) lower pro‐ and anti‐inflammatory cytokine production compared with control (A10). The allergenic fractions were characterised using an LC / MS / MS , which identified nine proteins. Among these, six proteins (tropomyosin, arginine kinase, haemocyanin, enolase, vitellogenin and 14‐3‐3 zeta) have been established as allergenic in prawn muscle and ovaries. Other three proteins (beta‐1,3‐glucan‐binding protein, translationally controlled tumour protein and farnesoic acid O‐methyltransferase short isoform protein) identified in this study need further investigation for their immunogenic properties.