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Application of Cudrania tricuspidata leaf extract as a washing agent to inactivate Listeria monocytogenes on fresh‐cut romaine lettuce and kale
Author(s) -
Woo HyukJe,
Kang JiHoon,
Lee ChaeHun,
Song Kyung Bin
Publication year - 2020
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.14305
Subject(s) - listeria monocytogenes , antimicrobial , biology , food science , preservative , botany , chemistry , microbiology and biotechnology , bacteria , genetics
Summary The present study aimed to examine the antimicrobial activity of silkworm thorn ( Cudrania tricuspidata ) leaf extract (CTLE) and its application as a washing agent for fresh‐cut romaine lettuce and kale. The antimicrobial activities of CTLE at various concentrations against Listeria monocytogenes were determined using disc diffusion and viable cell count assays, and CTLE exhibited strong inactivation of L. monocytogenes . In particular, after the treatment with 0.4% CTLE, the protein content of L. monocytogenes cells was significantly ( P < 0.05) decreased from 507.21 to 254.58 μg mL −1 , and damaged cells were observed by scanning electron microscopy. CTLE washing resulted in approximately 2‐log reduction of microbial growth, similar to that of 0.01% NaOCl. In addition, CTLE washing did not affect the quality of romaine lettuce and kale. Therefore, these results suggest that CTLE is applicable as a new washing agent for inactivating L. monocytogenes on fresh‐cut romaine lettuce and kale.