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Synergistic antimicrobial effect of lactocin AL 705 and nisin combined with organic acid salts against Listeria innocua 7 in broth and a hard cheese
Author(s) -
Verdi María Clara,
Melian Constanza,
Castellano Patricia,
Vignolo Graciela,
Blanco Massani Mariana
Publication year - 2020
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.14302
Subject(s) - nisin , potassium sorbate , chemistry , antimicrobial , food science , listeria , listeria monocytogenes , propionate , sodium propionate , sorbic acid , bacteriocin , microbiology and biotechnology , bacteria , biochemistry , organic chemistry , biology , genetics , sugar
Summary The effectiveness of antimicrobial mixtures against Listeria innocua 7, used as a L. monocytogenes surrogate, was investigated in broth and a food system. Synergistic effects were found for nisin (Nis), potassium sorbate ( PS ), calcium propionate ( CP ) and sodium lactate ( SL ), Nis + PS being the most effective binary mixture that exhibited listericidal activity in broth. To assess the effect of adding lactocin AL 705 ( AL 705) to Nis + organic acid salt combinations, tridimensional isobolograms were generated. Sub‐ MIC combinations of the antimicrobials exerted bactericidal activity against L. innocua 7 after AL 705 addition to the binary mixtures. However, when applied on Sardo cheese contaminated with L. innocua 7 (initial inoculum 4.45 ± 0.06 CFU g −1 ), only Nis + PS + AL 705 produced count reductions respect to the control, reaching 3.04 ± 0.35 CFU g −1 counts after 15 days at 15 °C. Ternary combinations containing AL 705 showed potential to reduce antimicrobial usages for L. innocua 7 inhibition.