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Inhibition of selected pathogens inoculated on the surface of catfish fillets by high molecular weight chitosan coating
Author(s) -
Karsli Baris,
Caglak Emre,
Li Dapeng,
Rubio Nancy K.,
Janes Marlene,
Prinyawiwatkul Witoon
Publication year - 2019
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.13897
Subject(s) - vibrio parahaemolyticus , chitosan , listeria monocytogenes , catfish , microbiology and biotechnology , antimicrobial , food science , salmonella , escherichia coli , staphylococcus aureus , chemistry , inoculation , listeria , vibrio , bacteria , biology , fish <actinopterygii> , fishery , biochemistry , genetics , gene , immunology
Summary Antibacterial activity of high molecular weight water‐soluble (HMWWS) chitosan (800 kDa) was investigated against four Gram‐negative ( Escherichia coli, Salmonella typhimurium , Vibrio cholerae and Vibrio parahaemolyticus ) and two Gram‐positive ( Staphylococcus aureus and Listeria monocytogenes ) bacteria. Catfish fillets were surface‐inoculated with these food‐borne pathogens and coated with chitosan dissolved in aspartic acid (AS) or acetic acid (AC) solution at different concentrations (1% or 3%). Samples were stored at 4 °C for 8 days, except for those inoculated with Vibrio species (10 °C for 6 days). Overall, the most effective coating treatment was the 3% HMWWS chitosan in AS solution (800AS3%). Compared with the control, significant ( P < 0.05) reductions caused by 800AS3% were observed for all tested pathogens at the end of storage. The growth of V. parahaemolyticus was completely suppressed by 800AS3%. This study demonstrated that HMWWS chitosan in AS solution could be used as an alternative antimicrobial coating for catfish fillets.