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Development and validation of a multiplex PCR assay for simultaneous detection of chicken , turkey and duck in processed meat products
Author(s) -
Kim MiJu,
Yoo Insuk,
Yang SeungMin,
Suh SeungMan,
Kim HaeYeong
Publication year - 2018
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.13876
Subject(s) - primer (cosmetics) , multiplex polymerase chain reaction , multiplex , biology , gene , microbiology and biotechnology , positive control , food science , polymerase chain reaction , genetics , chemistry , medicine , organic chemistry , traditional medicine
Summary We developed a multiplex PCR assay for the efficient detection of chicken , turkey and duck meat. Three species‐specific primer sets targeting 12S rRNA gene of turkey and mitochondrial cytochrome b ( CYTB ) gene of chicken and duck were selected. A universal primer pair targeting 18S rRNA gene was additionally used as a positive control. PCR product sizes for endogenous control, chicken, turkey and duck were confirmed to be 99 bp, 133 bp, 163 bp and 204 bp respectively. The specificity of each primer pairs was evaluated in 20 animal species. The limit of detection of this multiplex PCR assay was 1 pg for all species. Furthermore, this method was successfully applied to 31 commercial meat samples and validated at intra‐laboratory. This multiplex PCR assay for the simultaneous identification of chicken, turkey and duck meat in processed products can be utilised to monitor various processed food products for food control and regulation.