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Comparison of DNA extraction methods for entomological origin identification of honey using simple additive weighting method
Author(s) -
Kek Siok Peng,
Chin Nyuk Ling,
Tan Sheau Wei,
Yusof Yus Aniza,
Chua Lee Suan
Publication year - 2018
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.13840
Subject(s) - dna extraction , 16s ribosomal rna , amplicon , dna , genomic dna , biology , extraction (chemistry) , mitochondrial dna , chromatography , gene , polymerase chain reaction , computational biology , microbiology and biotechnology , genetics , chemistry
Summary Four different DNA protocols were compared to obtain the best method for DNA extraction of bees in honey samples. The efficiency was evaluated in terms of DNA concentration and purity, PCR amplification capacity targeting mitochondrial 16S rRNA gene, and execution time. The most efficient DNA extraction method was selected using simple additive weighting ( SAW ) analysis. The DN easy method with silica membrane spin‐column format scored highest in the final ranking of SAW compared to CTAB ‐based, Wizard and NucleoSpin methods. It yielded extracted DNA with sufficient concentration (second highest), acceptable purity (1.5–2.0), amplifiable for 16S rRNA gene region, and required least extraction time. This recommended DNA extraction protocol suggests that genomic methodologies using a 150 bp amplicon of the 16S rRNA gene of bees enables identification of the entomological origin of honey.