Premium
Rapid detection and toxicity assessment of citreoviridin using luminescent Vibrio qinghaiensis sp.‐Q67 in drinking water
Author(s) -
Wu Shizheng,
Zhang Xuhui,
Yang Panpan,
Li Liang,
Tang Shuze
Publication year - 2018
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.13801
Subject(s) - chemistry , vibrio , mycotoxin , food science , chromatography , bacteria , biology , genetics
Summary Citreoviridin ( CIT ), one of the mycotoxins produced by Penicillium citreoviridin ( PCV ), is a common contaminant in a wide range of agricultural products and is detrimental to human and animal health. There is potential threat that terrorists can extract CIT from mouldy cereals, such as rice which forms the major source of starch intake in Asia–Pacific diets and corns which are readily available. The objective of this study was to develop a rapid method for detection of citreoviridin and evaluate its performance on luminescent bacterium Vibrio qinghaiensis sp.‐Q67 (Q67). A good correlation ( R 2 > 0.98) between concentrations of CIT and the relative luminosity unit of Q67 was obtained with a linear range from 0.0005 to 0.01 mg L −1 . The precision expressed as relative standard deviation ( RSD ) was 3.2% for eleven replicate determinations of 0.005 mg L −1 CIT , and the recoveries of spiked samples were 88%–93%. The luminescence inhibition ratio of CIT increased from 0.0005 to 0.5 mg L −1 , and the luminescence inhibition ratio remained stable at approximately 85% in the range of 0.5–10 mg L −1 . The system detected low traces (≤0.0005 mg L −1 ) of CIT , and it has potential to be used as rapid detection method for mycotoxins. The effect of pure CIT on Q67 was time dependent and, it was the strongest ( EC 50 = 0.0066) at 20 min which indicated the high effect of CIT on Q67. Results obtained in this study suggest that Q67 is a promising method for rapid evaluation of the toxicity of CIT . Therefore, the luminescent bacteria assay can potentially be used as a system in monitoring sudden pollution in drinking water.