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Antioxidant activity in HepG2 cells, immunomodulatory effects in RAW 264.7 cells and absorption characteristics in Caco‐2 cells of the peptide fraction isolated from Dendrobium aphyllum
Author(s) -
Liu Huifan,
Ye Hongji,
Sun Chongzhen,
Xi Hongru,
Ma Juanjuan,
Lai Furao,
Wu Hui
Publication year - 2018
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.13783
Subject(s) - antioxidant , caco 2 , absorption (acoustics) , peptide , chemistry , immune system , in vitro , monolayer , biochemistry , cell , biology , immunology , materials science , composite material
Summary DA ‐P, fraction of peptides with a molecular weight <1 kD a isolated from Dendrobium aphyllum , was analysed in three types of cell lines to verify its bioactivity and absorptivity. The cellular antioxidant activity of DA ‐P in HepG2 cells was used and results revealed an EC 50 of 2.88 ± 0.143 mg mL −1 and a CAA unit of 63.46 ± 2.11 μ m QE /100 g peptides. DA ‐P treatment enhanced the secretion of cytokines in RAW 264.7 cells. After demonstrating the presence of tight junctions in Caco‐2 monolayers, the absorption was 25.57% ± 0.016% and 19.7% ± 0.012% from different sides. The relatively high absorption indicated that the antioxidant‐relevant immune functions of DA ‐P had a greater possibility to be absorbed by Caco‐2 cells. Free amino acids and LC ‐ MS / MS analysis indicated the degradation and expulsion of components after the absorption of DA ‐P, and Ser‐Ser‐Arg was able to come across the monolayers.