Structural characterisation and immunomodulatory effects of polysaccharides isolated from Dendrobium aphyllum

Summary A crude polysaccharide extract of Dendrobium aphyllum ( cDAP , yield 38.15 ± 0.20%) was generated. The D .  aphyllum polysaccharide ( DAP , M w 471.586 kD a), purified by DEAE ‐Sepharose and Sephadex‐G200 Fast Flow, was composed of mannose (71.3%) and glucose (28.7%), according to GC – MS analysis. Its backbone was composed of β‐ d ‐mannopyranose and β‐ d ‐glucopyranose residues, as revealed by infrared spectroscopic analysis. Its glycosidic bond was mainly 1, 4‐linked, and the O ‐acetyl groups were mainly linked to mannose residues, according to periodate oxidation and Smith degradation analysis. The DAP units polymerised into a filiform‐shaped spatial pattern, as characterised by atomic force microscopy and scanning electron microscopy. DAP treatment enhanced cytokine secretion (nitric oxide, interleukin‐6 and tumour necrosis factor‐α) and pinocytic and phagocytic capacities of RAW 264.7 mouse macrophages. The complement receptor 3 and mannose receptor were identified to be the receptors of DAP on RAW 264.7 cells, indicating that the Akt/ mTOR / MAPK and IKK /nuclear factor‐ ĸB pathways could be involved in DAP ‐activated immunomodulation.