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Contribution of different molecular weight fractions to anticancer effect of sweet potato protein hydrolysates by six proteases on HT ‐29 colon cancer cells
Author(s) -
Zhang Miao,
Mu TaiHua
Publication year - 2018
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.13625
Subject(s) - proteases , apoptosis , hydrolysate , chemistry , cell cycle , cell , enzyme , cancer cell , centrifugation , biochemistry , microbiology and biotechnology , cancer , biology , hydrolysis , genetics
Summary The contribution of different molecular weight fractions to anticancer effect of sweet potato protein hydrolysates ( SPPH ) by six proteases on HT ‐29 colon cancer cells was investigated. SPPH prepared by six proteases showed certain antiproliferation effect on HT ‐29 cells. Compared with other five proteases, SPPH by Alcalase exhibited the highest antiproliferation effect with the lowest IC 50 value of 119.72 μg mL −1 . SPPH by Alcalase was further separated into four fractions (>10, 5–10, 3–5 and <3 kD a), and <3 kD a fractions showed the strongest antiproliferation effect, which was 43.87% at 100 μg mL −1 ( P < 0.05). The <3 kD a fractions could cause G2/M cell cycle arrest with increased p21 expression and induce apoptosis via decreasing Bcl‐2 expression, increasing Bax expression and inducing caspase‐3 activation in HT ‐29 cells. In addition, <3 kD a fractions could significantly inhibit cell migration of HT ‐29 cells. Thus, SPPH might be potentially used as a natural supplement in functional foods.