Premium
Broccoli seed extracts but not sulforaphane have strong free radical scavenging activities
Author(s) -
Ligen Zou,
Yuanfeng Wu,
Yuke Shen,
Lei Zhang,
Mupunga Jothame,
Jianwei Mao,
Shiwang Liu
Publication year - 2017
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.13521
Subject(s) - sulforaphane , chemistry , dpph , scavenging , abts , ascorbic acid , antioxidant , chemiluminescence , radical , food science , chromatography , biochemistry
Summary The abilities of broccoli seed extracts and purified sulforaphane ( SF ) to scavenge 2,2′‐azinobis [3‐ethylbenzothiazoline‐6‐sulphonate] ( ABTS •+ ), 2,2‐diphenyl‐1‐picrylhydrazyl ( DPPH • ) and superoxide anions were studied. The free radical scavenging activities of broccoli seed extracts had no exact correlation with SF content, indicating that SF was not the main antioxidant component. The broccoli seed extracts were separated by high‐speed countercurrent chromatography to obtain purified SF , which showed dose‐dependent free radical scavenging activities in the DPPH • and ABTS •+ assays, but its activities were weaker to those of ascorbic acid. The same trend was observed in the chemiluminescence assay. The results showed that broccoli seed extracts had strong free radical scavenging activities that were not attributable to SF .