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Hypolipidaemic and antioxidant capacities of polysaccharides obtained from Laminaria japonica by different extraction media in diet‐induced mouse model
Author(s) -
Yao Yujing,
Xiang Huan,
You Lijun,
Cui Chun,
SunWaterhouse Dongxiao,
Zhao Mouming
Publication year - 2017
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.13508
Subject(s) - citric acid , antioxidant , chemistry , polysaccharide , trolox , abts , food science , dry weight , phosphoric acid , extraction (chemistry) , oral administration , biochemistry , dpph , chromatography , pharmacology , botany , biology , organic chemistry
Summary This study shows the industrial feasibility of using aqueous methods to produce antioxidative and hypolipidaemic polysaccharides from Laminaria japonica ( LJP ). Comparison was firstly made among the polysaccharides prepared using different extraction media, that is water alone ( LJPW ) and citric acid ( LJPC ), sulphuric acid, hydrochloric acid and phosphoric acid. LJPC enabled the highest extract yield (~11% dry weight), bile salt adsorption rate (~59% dry weight), ABTS radical scavenging activity ( IC 50 value 1.06 mg mL −1 ) and ORAC antioxidant activity (341.87 μmol Trolox g −1 ). In animal trial using diet‐induced high‐fat mice, oral administration of LJP produced with citric acid ( LJPC ) at a high dose (200 mg kg −1 body mass per day) enabled significantly higher serum HDL ‐C, lower LDL ‐C/ HDL ‐C and unaltered LDL ‐C, whilst a medium dose (100 mg kg −1 body mass per day) significantly decreased LDL ‐C. Administration of LJP produced with water (200 mg kg −1 body mass per day) significantly lowered serum LDL ‐C. Therefore, LJP may provide dose‐dependent pharmacological and therapeutic effects to combat atherosclerosis through their hyperlipidaemic and antioxidant properties.