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Analysis of solvent effects on polyphenols profile, antiproliferative and antioxidant activities of mulberry ( Morus alba L.) extracts
Author(s) -
Li Fuhua,
Zhang Bin,
Chen Gu,
Fu Xiong
Publication year - 2017
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.13443
Subject(s) - chemistry , acetic acid , acetone , antioxidant , polyphenol , solvent , ethanol , flavonols , methanol , extraction (chemistry) , yield (engineering) , food science , nuclear chemistry , biochemistry , organic chemistry , materials science , metallurgy
Summary A systematic study about the solvent effect on the mulberry polyphenol ( MP ) profile, antioxidant and antiproliferation capacities against human hepatoma HepG2 cells was conducted. Results indicated that MP profiles showed significant differences as a function of solvents including AA /W (acetic acid/water), Me OH / AA /W (methanol/ acetic acid/water), Et OH / AA /W (ethanol/ acetic acid/water) and ME 2 CO / AA /W (acetone/ acetic acid/water). Among the solvents, Et OH / AA /W took advantage in obtaining MP with the highest yield (49.81 mg GAE g −1 dw), and cellular antioxidant capacity in the PBS no‐wash protocol (63.2 μmolQE/100 g), as well as low cell cytotoxicity (≥50.0 mg mL −1 ). ME 2 CO / AA /W was the best choice for extracting MP with the most various compositions (two phenolic acids, four anthocyanins and four flavonols), greatest potential in inhibiting proliferation of HepG2 cells ( EC 50 = 28.2 mg mL −1 ) and highest intracellular antioxidant activity (38.0 μmol QE /100 g). Results provide baseline information for efficient solvent extraction to obtain MP that is promising as natural antioxidants and antitumor agents.