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The chelating peptide ( GPAGPHGPPG ) derived from Alaska pollock skin enhances calcium, zinc and iron transport in Caco‐2 cells
Author(s) -
Chen Qianru,
Guo Lidong,
Du Fen,
Chen Tiejun,
Hou Hu,
Li Bafang
Publication year - 2017
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.13396
Subject(s) - chemistry , chelation , calcium , peptide , zinc , in vitro , caco 2 , digestion (alchemy) , metal ions in aqueous solution , histidine , biochemistry , metal , enzyme , chromatography , inorganic chemistry , organic chemistry
Summary Gly‐Pro‐Ala‐Gly‐Pro‐His‐Gly‐Pro‐Pro‐Gly ( GPAGPHGPPG ), a chelating peptide derived from Alaska pollock skin, has been approved with outstanding ability to chelate calcium, zinc and iron ions. In this study, the stability of GPAGPHGPPG during in vitro g astrointestinal enzymatic digestion, the potential binding site between peptide and metal ions as well as its effects on mineral transport in Caco‐2 cells were investigated. Results showed that approximately 75% of GPAGPHGPPG remained intact at the end of the in vitro g astrointestinal enzymatic digestion. The formation of peptide‐metal complex was potentially related to histidine and cyclic structure in terms of dehydration. Moreover, GPAGPHGPPG revealed significant promotional effects on calcium (112.7%, P < 0.01), zinc (32.3%, P < 0.01) and iron (27.7%, P < 0.01) transport in Caco‐2 cell monolayer. In conclusion, GPAGPHGPPG could be developed as a potential functional ingredient to prevent mineral deficiency.