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DNA barcoding for the species identification of commercially important fishery products in Indonesian markets
Author(s) -
Abdullah Asadatun,
Rehbein Hartmut
Publication year - 2017
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.13278
Subject(s) - dna barcoding , genbank , biology , species identification , identification (biology) , barcode , cytochrome c oxidase subunit i , indonesian , molecular marker , fishery , mitochondrial dna , gene , evolutionary biology , ecology , genetics , business , linguistics , philosophy , marketing
Summary The DNA barcoding approach was used for the species identification of 44 Indonesian commercial fishery products. Additionally, the intronless nuclear rhodopsin gene fragment ( RH 1 ) was added to the analysis to enable the identification of species not yet barcoded and possible hybrids. The 655‐bp cytochrome C oxidase subunit I ( COI ) gene fragment marker was successfully amplified and used to identify 86% of the total fish samples at the species level using the BOLD and BLAST public databases. Moreover, the RH 1 marker was used to complete COI analysis. For a number of fish species, the COI sequences (six species) and RH 1 sequences (eight species) were the first entries submitted to GenBank. This study demonstrated that COI barcoding is a promising tool for Indonesian fishery products and confirmed that it could be adopted in the future for regular seafood control as part of the Indonesian integrated food traceability system.