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Phytosterols in banana ( Musa spp .) flower inhibit α‐glucosidase and α‐amylase hydrolysations and glycation reaction
Author(s) -
Sheng Zhanwu,
Dai Haofu,
Pan Siyi,
Ai Binling,
Zheng Lili,
Zheng Xiaoyan,
Prinyawiwatkul Witoon,
Xu Zhimin
Publication year - 2017
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.13263
Subject(s) - glycation , amylase , chemistry , sugar , blood sugar , fructose , enzyme , food science , reducing sugar , diabetes mellitus , biochemistry , endocrinology , biology , receptor
Summary Three phytosterols were isolated from Musa spp . flowers for evaluating their capabilities in inhibiting glucosidase and amylase activities and glycation of protein and sugar. The three phytosterols were identified as β‐sitosterol (PS1), 31‐norcyclolaudenone (PS2) and (24R)‐4α, 14α, 4‐trimethyl‐5α‐cholesta‐8, 25(27)‐dien‐3β‐ol (PS3). IC 50 values (the concentration of inhibiting 50% of enzyme activity) of PS1, PS2 and PS3 against α‐glucosidase were 283.67, 11.33 and 43.10 μg mL −1 , respectively. For inhibition of α‐amylase, the IC 50 values of PS1, PS2 and PS3 were 52.55, 76.25 and 532.02 μg mL −1 , respectively. PS1 was an uncompetitive inhibitor against α‐amylase with K m at 5.51 μg mL −1 , while PS2 and PS3 exhibited a mixed‐type inhibition with K m at 52.36 and 2.49 μg mL −1 , respectively. PS1 and PS2 also significantly inhibited the formation of advanced glycation end products (AGEs) in a BSA–fructose model. The results suggest that banana flower could possess the capability in prevention of the diseases associated with abnormal blood sugar and AGEs levels, such as diabetes.