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Antioxidant and hypoglycaemic effects of tilapia skin collagen peptide in mice
Author(s) -
Zhang Ruilin,
Chen Jian,
Jiang Xiongwu,
Yin Lasheng,
Zhang Xuewu
Publication year - 2016
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.13193
Subject(s) - antioxidant , chemistry , tilapia , dpph , metformin , diabetes mellitus , abts , pharmacology , endocrinology , medicine , biochemistry , biology , fishery , fish <actinopterygii>
Summary In this study, tilapia collagen peptide ( TCP ) was prepared by alcalase hydrolysis of tilapia skin, and the antioxidant and hypoglycaemic effects of TCP were investigated. The results showed that TCP possessed DPPH and ABTS + free radicals‐scavenging activities. In diabetic mice in which diabetes was induced by injection of alloxan (50 mg kg −1 bw), high‐dose TCP (1.7 g kg −1 bw) and the drug metformin (1.0 g kg −1 bw) were found to reduce 31.8% and 30.3% of blood glucose levels in 25 days, respectively. Moreover, in diabetic mice receiving high‐dose TCP , antioxidant enzymes SOD and CAT were increased by 23% and 59.2%, respectively, and MDA was decreased by 39.1%. Comparing the treated high‐dose TCP group with the metformin group, there were similar SOD (61.5 U mg −1 vs. 60.2 U mg −1 ) and MDA (1.4 nmol mg −1 vs. 1.3 nmol mg −1 ), but more (~7%) CAT (359.8 U g −1 vs. 336.1 U g −1 ). Together, the present data, for the first time, demonstrated that TCP possessed hypoglycaemic effects in mice.