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Assessment of the ability of five culture media for the detection of E scherichia coli O157
Author(s) -
Gutierrez Myriam E.,
Janes Marlene E.,
Torrico Damir D.,
Carabante Kennet M.,
Prinyawiwatkul Witoon
Publication year - 2016
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.13164
Subject(s) - agar , cefixime , escherichia coli , microbiology and biotechnology , tryptic soy broth , isolation (microbiology) , agar plate , biology , feces , food science , bacteria , veterinary medicine , gene , biochemistry , medicine , genetics , cephalosporin , antibiotics
Summary Cattle are a common reservoir for Escherichia coli O157:H7. Prior to confirming its presence in a sample, proper isolation of E. coli O157 is necessary. Consequently, this study evaluated the ability of five commercial plating media to isolate E. coli O157 from 138 samples of fresh cattle faeces, water from water trough and pond, and surfaces of water trough and hay bunk. For the isolation of E. coli O157, samples were enriched in tryptic soya broth, followed by immunoseparation and then plating on SMAC , CT ‐ SMAC , CHROM agar ™ O157, Tellurite CHROM agar ™ O157 and Vancomycin Cefixime Cefsoludin CHROM agar ™ O157. Real‐time PCR targeting genes stx 1 , stx 2 and wzy O157 was used to confirm selected isolates. When analysed together, CT ‐ SMAC and CHROM agar ™ O157 were the best combination for isolating E. coli O157, giving 79% true‐positive results and only 0.05% false‐negative results.