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Effects of acid concentration and the UHP pretreatment on the gelatinisation of collagen and the properties of extracted gelatins
Author(s) -
Zhang Yuhao,
Ma Liang,
Cai Luyun,
Zhou Mengrou,
Li Jianrong
Publication year - 2016
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.13089
Subject(s) - gelatin , thermostability , chemistry , fourier transform infrared spectroscopy , covalent bond , nuclear chemistry , polymer chemistry , biochemistry , enzyme , chemical engineering , organic chemistry , engineering
Summary An ultra‐high‐pressure ( UHP ) transmission medium with HC l was applied as a pretreatment to extract gelatin. The effects of the acid concentration (0–1% (w/v)) on the gelatinisation of collagen and the properties of gelatin were investigated. An increase in the acid concentration decreased the thermostability of collagen and increased the yield of gelatin (from 64% increased to 80%). The content of the subunit components in the pretreated collagen and the gelatin declined slightly as the acid concentration was increased, resulted in slight decrease ( P > 0.05) in the gel strength (from 435 g decreased to 408 g). The analysis of Fourier transform infrared spectroscopy ( FTIR ) spectra showed that the triple‐helical structure, secondary structures of the collagen and covalent cross‐linking in the collagenous fibres were damaged gradually as the acid concentration was increased. The scanning electron microscopy ( SEM ) images showed that the smooth surface of collagen was partly disrupted with microvoids after UHP /1% HC l pretreatment, which may be related to the damage on the covalent cross‐linking.