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Effects of pulsed electric fields on the survival behaviour of Saccharomyces cerevisiae suspended in single solutions of low concentration
Author(s) -
Wang ManSheng,
Zeng XinAn,
Brennan Charles S.,
Brennan Margaret A.,
Han Zhong
Publication year - 2016
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.13007
Subject(s) - saccharomyces cerevisiae , intensity (physics) , electric field , pulse (music) , materials science , chemistry , analytical chemistry (journal) , food science , biophysics , biology , yeast , chromatography , biochemistry , optics , physics , quantum mechanics , detector
Summary This paper investigates the survival behaviour of Saccharomyces cerevisiae cells suspended in NaCl solutions with various low concentrations after exposure to pulsed electric field ( PEF ) treatments. The input and heat‐generation pulse power during each PEF treatment experiment were measured directly. In addition, detailed information on sublethally PEF ‐injured S. cerevisiae cells was obtained by the use of image analysis. Results showed that both the field intensity and the total treatment time had significantly affected the survival behaviour of S. cerevisiae cells, whereas the concentration of NaCl solution had less effect. When the S. cerevisiae cells were suspended in the solution with conductivity of 300 μS cm −1 , a high ratio of surviving cells was observed. Image analysis indicated a large number of sublethally injured cells ( SIC s) after the PEF treatment with low field intensity. Results indicate that the survival behaviour of cells suspended in solutions of low concentration was influenced by PEF intensity.