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Characteristics of collagen from the skin of clown featherback ( Chitala ornata )
Author(s) -
Kittiphattanabawon Phanat,
Benjakul Soottawat,
Sinthusamran Sittichoke,
Kishimura Hideki
Publication year - 2015
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.12864
Subject(s) - hydroxyproline , pepsin , glycine , chemistry , histidine , proline , biochemistry , lysine , isoelectric point , amino acid , imino acid , enzyme
Summary Acid soluble collagen ( ASC ) and pepsin soluble collagen ( PSC ) from the skin of clown featherback ( Chitala ornata ) were isolated and characterised. Yields of ASC and PSC were 27.64 and 44.63% (dry weight basis) with total collagen recovery of 82.08%. Both collagens contained glycine as the major amino acid with relatively high content of proline, hydroxyproline and glutamic acid/glutamine. Nevertheless, they had the low content of cysteine, histidine and tryrosine. The collagen was characterised as type I, comprising (α1) 2 α2‐heterotrimer. Pepsin‐aided process did not affect triple‐helical structure of PSC as determined by FTIR spectra. Thermal transition temperature of ASC (36.28 °C) was slightly higher than that of PSC (35.23 °C). However, no differences in isoelectric point (5.54–5.68) between ASC and PSC were observed. Therefore, collagen from the skin of clown featherback could be successfully extracted for further applications.