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Isolation of antimicrobial agent from the marine algae Cystoseira hakodatensis
Author(s) -
Jun JoonYoung,
Nakajima Sosuke,
Yamazaki Koji,
Kawai Yuji,
Yasui Hajime,
Konishi Yasuyuki
Publication year - 2015
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.12719
Subject(s) - antimicrobial , bacillus cereus , cereus , brown algae , phytochemical , bacillus licheniformis , algae , biology , chemistry , extraction (chemistry) , microbiology and biotechnology , food science , botany , traditional medicine , bacteria , chromatography , bacillus subtilis , genetics , medicine
Summary Cystoseira hakodatensis is an unutilised brown algae belonging to family Sargassaceae. A crude methanol extract from the algae showed inhibitory effects on the growths of Bacillus cereus and Bacillus licheniformis . To isolate the major antimicrobial agent, a sequential active‐guided isolation procedure was applied: liquid–liquid extraction, column chromatography and bio‐autography. A marked antimicrobial agent (active α) was isolated in hydrophobic fraction and was determined to phenolics without carbohydrates and proteins by phytochemical test. Regarding the antimicrobial potential, the isolated active α showed better inhibitory effects against B. cereus and B. licheniformis at 2 and 4 times of lower concentrations (62.5 and 31.3 μg mL −1 ) in comparison with epigallocatechin gallate. These results showed that C. hakodatensis is a potential source of antimicrobial agent capable of preventing the growth of the two bacteria.

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