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Acid‐soluble and pepsin‐soluble collagens from grass carp ( Ctenopharyngodon idella ) skin: a comparative study on physicochemical properties
Author(s) -
Chen Ye,
Ye Ran,
Wang Yan
Publication year - 2015
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.12675
Subject(s) - grass carp , pepsin , chemistry , solubility , differential scanning calorimetry , extraction (chemistry) , biochemistry , food science , chromatography , biology , organic chemistry , enzyme , fish <actinopterygii> , physics , fishery , thermodynamics
Summary In this study, acid‐soluble (ASC) and pepsin‐soluble (PSC) collagens with triple helical structures were successfully extracted from the skin of grass carp ( Ctenopharyngodon idella ) by two different extraction approaches. SDS‐PAGE pattern revealed that ASC and PSC are type I collagens with typical α1, α2 and β‐chains. In addition, the intensity of χ‐chain (trimer) in ASC was higher than that of PSC, representing the presence of the high proportion of intra‐ and intermolecular cross‐links of extracted collagens with large molecular weight using the acid method. Differential scanning calorimetry (DSC) results demonstrate that T d (69.04 °C) of ASC was higher than T d (62.20 °C) of PSC. Both ASC and PSC had the highest solubility at acidic pH s or at a low concentration of NaCl (<2%, w/v). The results of FTIR suggested the ASC and PSC maintained in the helical secondary structure at high degree.