Premium
Quantitative UPLC ‐ MS / MS analysis of chlorogenic acid derivatives in antioxidant fractionates from dandelion ( Taraxacum officinale ) root
Author(s) -
Kenny Owen,
Smyth Thomas J.,
Hewage Chandralal M.,
Brunton Nigel P.
Publication year - 2015
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.12668
Subject(s) - dandelion , chlorogenic acid , taraxacum officinale , antioxidant , chemistry , trolox , dpph , ethyl acetate , chromatography , fractionation , food science , biochemistry , medicine , alternative medicine , pathology , traditional chinese medicine
Summary While qualitative studies have identified chlorogenic acids in antioxidant extracts, particularly ethyl acetate‐derived extracts, of Taraxacum officinale , quantitative analysis of these phenolic compounds remains largely unreported for this species. In this study, bioactivity‐guided fractionation of an antioxidant crude ethyl acetate extract ( DPPH = 295.481 ± 0.955 mg TE g −1 extract) from T. officinale root resulted in a number of reverse‐phase fractions that demonstrated high antioxidant activity ( DPPH = 1058.733–1312.136 mg TE g −1 extract), stronger than that of the synthetic antioxidant Trolox ® . UPLC ‐ MS / MS screening of these fractions for the presence of selected mono‐ and di‐caffeoylquinic acids revealed large quantities of 1,5‐dicaffeoylquinic acid present in several fractions (853.052–907.324 μg mg −1 ), respectively. Due to the antioxidant potency and high levels of 1,5‐dicaffeoylquinic acid observed in these fractions, it was concluded that specifically this chlorogenic acid derivative is a major contributor to the antioxidant efficacy of dandelion root.