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In vitro anti‐hydroxyl radical activity of the fructooligosaccharides 1‐kestose and nystose using spectroscopic and computational approaches
Author(s) -
Pejin Boris,
Savic Aleksandar G.,
Petkovic Milena,
Radotic Ksenija,
Mojovic Milos
Publication year - 2014
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.12445
Subject(s) - hydroxyl radical , chemistry , electron paramagnetic resonance , oligosaccharide , natural product , antioxidant , in vitro , organic chemistry , biochemistry , nuclear magnetic resonance , physics
Summary Fructooligosaccharides ( FOS ) are bioactive oligosaccharide fructans with beneficial health effects. Anti‐hydroxyl radical activity is an important factor in the description of antioxidant capacity of any natural product. The aim of this study was to estimate in vitro anti‐hydroxyl radical activity of the FOS 1‐kestose and nystose by electron paramagnetic resonance spectroscopy ( EPR ) and fluorescence spectroscopy ( FS ) followed by a theoretical approach based on quantum chemistry calculations. A significant anti‐hydroxyl radical potential of both compounds was observed (72% and 78% by EPR and 69% and 74% by FS , respectively), indicating the nystose to be a more active natural product. In addition, the computational results have confirmed that nystose follows the same pattern previously shown for 1‐kestose, that is, that carbohydrates can react with hydroxyl radical. It is well known that FOS belong to cardioprotective nutraceuticals, so the study may be of some interest to research in heart disease.