Premium
Extraction, identification and enzymatic synthesis of acylated derivatives of anthocyanins from jaboticaba ( M yrciaria cauliflora ) fruits
Author(s) -
Castro Vanessa Cristina,
Silva Paulo Henrique Alves,
Oliveira Eduardo Basílio,
Desobry Stéphane,
Humeau Catherine
Publication year - 2014
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.12298
Subject(s) - ellagic acid , chemistry , abts , gallic acid , dpph , rutin , palmitic acid , glucoside , lipase , quercetin , chromatography , polyphenol , candida antarctica , acylation , organic chemistry , antioxidant , enzyme , fatty acid , medicine , alternative medicine , pathology , catalysis
Summary Polyphenols were extracted from the skin of jabuticaba fruits ( M yrciaria cauliflora ). Their total concentration and in vitro antioxidant activity were analysed by the DPPH and ABTS methods. The corresponding results (dry basis) were 1290 mg gallic acid equivalent ( GAE )∙(100 g) −1 , 98% of DPPH radical inhibition and 120 μ m TEAC ∙g −1 ( ABTS method). All these values are at least as higher as average values reported in the literature for other fruits. A more specific analysis of the fractions of phenolic compounds was also performed by HPLC ‐ MS . Ellagic acid, quercetin, rutin, delphinidin‐3‐glucoside and cyanidin‐3‐glucoside were the main compounds detected; the latter two were the most abundant. The crude extract was subjected to enzymatic acylation assays in order to synthesise new esters with new potential techno‐functionalities. Palmitic acid was used as acyl donor and lipase B of C andida antactica ( CALB ) as biocatalyst. HPLC ‐ MS evidenced the formation of palmitic monoesters in connection with the delphinidin‐3‐glucoside and cyanidin‐3‐glucoside fractions.