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Bifidobacterial growth–promoting effect of yak milk κ‐casein hydrolysates produced with different proteases
Author(s) -
Tang Xi,
Tian Qian,
Cheng Xue,
Li Nan,
Mao XueYing
Publication year - 2013
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.12138
Subject(s) - hydrolysate , casein , food science , papain , trypsin , chemistry , proteases , biochemistry , biology , enzyme , hydrolysis
Summary The growth‐promoting effects of κ‐casein hydrolysates produced by four different proteases (trypsin, alcalase, papain and chymosin) on bifidobacteria ( B b12 and BBMN 68) viability were evaluated. Results showed that the obtained κ‐casein hydrolysates possessed better property for improving the viability of B b12 and BBMN 68 than native κ‐casein. The bifidobacterium viability and p H drop were dependent on the added hydrolysates. The addition of κ‐casein hydrolysates improved the viability of B b12 and BBMN 68 to a variable extent. The κ‐casein hydrolysate produced with papain ( KCHP ) is the best for B b12 and κ‐casein hydrolysate produced with trypsin ( KCHT ) is the best for BBMN 68. Proper amino acid profiles improved the viability of B b12 and BBMN 68. The bifidobacterial growth‐promoting capacity of κ‐casein hydrolysate may be due to its high content of A la, M et, T yr, P he and A rg residues, especially T yr which is richer in para‐κ‐casein than in casein glycomacropeptide ( GMP ). In addition, the sialic acid content of κ‐casein hydrolysate produced with the tested proteases did not show a direct relationship with its bifidobacterial growth–promoting effect. The obtained peptides possessed growth‐promoting activity for B b12 and BBMN 68 existed in para‐κ‐casein.