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A simple preparative method for isolation and purification of polysaccharides from mulberry ( M orus alba L .) leaves
Author(s) -
Jia Dongdong,
Zhang Jun,
Lan Rong,
Yang Hongling,
Sun Yongyue
Publication year - 2013
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.12087
Subject(s) - polysaccharide , pectin , chemistry , ethanol precipitation , uronic acid , yield (engineering) , chromatography , adsorption , ethanol , extraction (chemistry) , gel permeation chromatography , sugar , precipitation , activated carbon , organic chemistry , polymer , food science , materials science , physics , meteorology , metallurgy
Summary An effective method for preparative extraction and purification of the polysaccharides from mulberry leaves was studied. Orthogonal experiment was carried out to get the optimal conditions ( T = 70 °C, pH = 6, t = 60 min, D = 0.4 g g −1 material) of activated carbon decolorisation. The staged, isocratic and multiple ethanol precipitation procedures were also investigated. Through the activated carbon decolorisation and 20% ethanol precipitation procedure, the polysaccharides yield reached 2.91%, and the content of neutral sugar and uronic acid were improved to 21.87% and 57.43% respectively. This process was superior to the macroporous resin adsorption and gel permeation chromatography ( GPC ) methods. The structure analysis indicated that the purified mulberry leaves polysaccharide ( MLP ) was composed of M an, G al A and R ha in the ratio of 1.0:7.2:20.0. The molecular weight measured using GPC was approximately 557 062 D. The IR and 1 H NMR spectra indicated that the MLP was mainly pectin, which was classified as high‐methoxyl pectin.