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Effects of polyphenol oxidation on in vitro iron availability in faba bean ( V icia faba L .) flour
Author(s) -
Luo YuWei,
Xie WeiHua
Publication year - 2013
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.12016
Subject(s) - polyphenol , polyphenol oxidase , food science , chemistry , incubation , tyrosinase , tannic acid , in vitro , green tea extract , antioxidant , biochemistry , enzyme , green tea , peroxidase , organic chemistry
Summary Tannic acid ( TA ) and green tea extract ( GTE ) were added to faba bean flour, before and after incubation with polyphenol oxidase (mushroom tyrosinase), and the effect on in vitro iron availability was investigated. The inhibitory effect of TA and GTE was dose dependent, and the in vitro iron availability decreased from 64.5% in the pure faba bean flour to 9.8% ( P < 0.05) with the addition of 1 mg of TA 1 g −1 to faba bean flour. The addition of 5 mg of GTE resulted in low in vitro iron availability as after the addition of 10 mg of TA (11.6% against 10.3% for 1 mg TA ). Incubation of the polyphenols with tyrosinase before addition to the faba bean flour significantly increased the in vitro iron availability. The increase in iron availability was significant ( P < 0.05) when amounts of 0.2, 0.5 or 1 mg g −1 TA or GTE added to faba bean flour, respectively, were oxidised, even with the lowest amount of tyrosinase (150 u). Oxidation of 1 mg TA or GTE with 150 u tyrosinase increased the in vitro iron availability from 10.3% to 15.5% and from 19.2% to 26.1%, respectively. At the 300‐u level, the addition of higher amounts of enzyme (from 600 to 900 u) did not have any effect. The results from the study therefore suggest that the oxidation of polyphenols may be a promising way to increase the availability of iron in polyphenol‐containing legume foods.