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High hydrostatic pressure inactivation kinetics of the endogenous lipoxygenase in crude silver carp ( H ypophthalmichthys molitrix ) extract
Author(s) -
Qiu Chunjiang,
Xia Wenshui,
Jiang Qixing
Publication year - 2013
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/ijfs.12000
Subject(s) - hydrostatic pressure , kinetics , chemistry , lipoxygenase , silver carp , reaction rate constant , chromatography , enzyme , biochemistry , thermodynamics , biology , physics , quantum mechanics , fishery , fish <actinopterygii>
Summary Inactivation kinetics of endogenous lipoxygenase in crude silver carp ( H ypophthalmichthys molitrix ) extract was studied for thermal (50–80 °C, ambient pressure) and high hydrostatic pressure combined with heat treatment (300–700 MP a at 50–80 °C) process conditions. Lipoxygenase ( LOX ) inactivation followed first‐order kinetics at all pressure–temperature combinations used. Moreover, LOX inactivation rates became less pressure dependent with increasing pressure at all temperatures studied and also showed less temperature dependence of the enzyme inactivation rate at various high pressure levels tested. It is evident that inactivation kinetics is complex affected by both pressure and temperature. Therefore, response surface methodology ( RSM ) was attempted to investigate combined temperature–pressure processing conditions on LOX inactivation kinetics in crude silver carp extract samples. In conclusion, the LOX inactivation rate constant was modelled as a function of both temperature and pressure conditions by a quadratic polynomial equation as follows: k = 0.278 − 3.991 E 004 · P − 0.01 · T + 6.167 E − 006 · P × T + 3.608 E − 007 P 2 + 9.525 E − 005 · T 2.