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Expression of Th17‐ and Treg‐specific transcription factors in vitiligo patients
Author(s) -
Bhardwaj Supriya,
Rani Seema,
Kumaran Muthu S.,
Bhatia Alka,
Parsad Davinder
Publication year - 2020
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1111/ijd.14766
Subject(s) - vitiligo , foxp3 , medicine , rar related orphan receptor gamma , immunology , transcription factor , peripheral blood mononuclear cell , population , cancer research , immune system , biology , gene , genetics , environmental health , in vitro
Background Vitiligo is mainly considered an autoimmune skin disease as the number of IL‐17 producing Th17 cells, involved in the development of autoimmune and inflammatory pathologies, increased in vitiligo skin. T regulatory cells (Tregs) seem to be altered during the disease. Thus, there must be some upstream molecular factors that regulate the cellular response to apoptotic and inflammatory stimuli. Objectives To investigate the expression of Th17‐ and Treg‐specific transcription factors in PBMCs and to evaluate the correlation between these transcription factors and cytokines in vitiligo patients. Methods We investigated 30 active NSV patients for Th17‐ and Treg‐specific transcription factors RORγt (retinoic acid‐related orphan receptor gamma t), FOXP3 (forkhead/winged helix), HELIOS, EOS, and IRF4 (Interferon Regulatory Factor 4) as well as apoptotic marker NALP1 (NACHT‐leucine‐rich‐repeat protein 1) in PBMCs with RT‐qPCR. Immunostaining was done for transcription factors and cytokines on skin sections. Results The mRNA level of FOXP3 was significantly lower in patients (0.76 fold, P < 0.001), whereas RORγt was slight but not significantly increased (0.76 fold, P = 0.06). Furthermore, NALP1 in lymphocytes was found to be increased in patients (0.69 fold, P < 0.01). The immunostaining results revealed increased expression of RORγ, IL‐17A, NALP1, and IL‐1β in vitiligo skin when compared to normal healthy skin. Conclusion Reduced FOXP3/RORγt mRNA ratio suggests thriving of the Th17 cell population in PBMCs of vitiligo patients. Increased NALP1 levels indicate the existence of an apoptotic phenomenon which correlates with the increased expression of IL‐1β in vitiligo pathogenesis.