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Levels of miR‐31 and its target genes in dermal mesenchymal cells of patients with psoriasis
Author(s) -
Wang Qiang,
Chang Wenjuan,
Yang Xiaohong,
Cheng Yueai,
Zhao Xincheng,
Zhou Ling,
Li Juan,
Li Junqin,
Zhang Kaiming
Publication year - 2019
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1111/ijd.14197
Subject(s) - psoriasis , pathogenesis , mesenchymal stem cell , medicine , gene expression , gene , microrna , pathology , cancer research , immunology , biology , genetics
Background Psoriasis is characterized by chronic inflammatory dermatosis, and the pathogenesis of psoriasis is associated with mesenchymal stem cells ( MSC s) and deregulation of the expression of miR‐31. This study aimed to clarify the function of miR‐31 in dermal MSC s ( DMSC s) in the pathogenesis of psoriasis. Methods The expression of miR‐31 was assayed by a microarray and that of target genes of miR‐31 was tested by quantitative PCR . Results The expression of miR‐31 in the psoriasis group was 0.2677 folds that of the control group. The expression of EMP 1 and EIG 121L genes, whose products are located on the cell membrane, in the psoriasis group was 4.095579 and 5.367017 folds that in the control group, respectively. The expression of GRB 10 , PTPN 14 , QKI , RNF 144B , and TACC 2 genes, whose products are located in the cytoplasm, in the psoriasis group was 1.440428, 1.198335, 1.737285, 7.379546, and 1.531947 folds that of the control. The expression of PRELP , whose products are secreted in the extracellular space, in the psoriasis group was 1.351684 folds that of the control. The expression of RBMS 1 , KHDRBS 3 , and SATB 2 , whose products play a role in the nucleus, in the psoriasis group was 2.237199, 1.277159, and 1.005742 folds that of the control, respectively. Conclusions Our results suggest that the low expression of miR‐31 in DMSC s in patients with psoriasis causes an increase in the expression of some of its target genes, which in turn facilitates T lymphocyte activation by inhibiting the proliferation of DMSC s and therefore participates in the pathogenesis of psoriasis.