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Expression levels of transcription factor PU .1 and interleukin‐9 in atopic dermatitis and their relation to disease severity and eruption types
Author(s) -
Hamza Ashraf M.,
Omar Salma S.,
Abo ElWafa Reham A. H.,
Elatrash Marwa J.
Publication year - 2017
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1111/ijd.13579
Subject(s) - atopic dermatitis , scorad , medicine , pathogenesis , immunoglobulin e , immunology , interleukin , asthma , psoriasis , dermatology life quality index , antibody , cytokine
Background The role of immunological factors in atopic dermatitis ( AD ) pathogenesis is well established. T‐helper ( TH ) cells are central in AD pathogenesis. A relatively new subset of T cells, Th9 cells, was shown to be involved in the development of allergic asthma and allergic rhinitis, while its role in AD is still to be investigated. This study aimed to measure gene expression levels of interleukin‐9 ( IL ‐9) and PU .1, and to examine relationships with disease severity, serum IgE, and eruption types in AD patients. Methods The study enrolled 30 AD patients, 30 psoriasis patients, and 30 healthy subjects. The severity of AD was assessed using the SCORAD index. IL ‐9 and PU .1 expressions were measured by using real‐time quantitative polymerase chain reaction ( RQ ‐ PCR ). Serum IgE was measured by IgE (human) enzyme‐linked immunosorbent assay ( ELISA ) Kit. Results IL ‐9 and PU.1 gene expressions were significantly higher in AD patients than in controls ( P 1 = 0.007, P 2 < 0.001, respectively). In the atopic dermatitis patients, expression of IL ‐9 and PU.1 were significantly positively correlated with SCORAD index ( P 1 = 0.004, P 2 = 0.002) and clinically with erythema and edema scores. IL ‐9 and PU .1 expressions were positively significantly correlated ( P = 0.005) and positively correlated with serum IgE in the AD group ( P 1 = 0.017, P 2 = 0.023). No significant difference was noted between AD patients with or without histories of other atopies regarding expression levels of IL ‐9 and PU.1 ( P 1 = 0.677, P 2 = 0.135). Conclusions PU .1 and IL ‐9 may play a role in AD pathogenesis and relate to disease severity and clinical eruption types.