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Phenotypic analysis of circulating T‐cell subset and its association with burden of skin disease in patients with chronic actinic dermatitis: a hematologic and clinicopathologic study of 20 subjects
Author(s) -
Hamada Toshihisa,
Aoyama Yumi,
Shirafuji Yoshinori,
Iwatsuki Keiji
Publication year - 2017
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1111/ijd.13486
Subject(s) - medicine , erythroderma , skin biopsy , dermatology , peripheral blood mononuclear cell , biopsy , pathology , etiology , mycosis fungoides , cutaneous t cell lymphoma , gastroenterology , lymphoma , biochemistry , chemistry , in vitro
Abstract Background Chronic actinic dermatitis ( CAD ) is a recurrent photosensitive dermatitis that occurs predominantly on sun‐exposed areas with unknown etiology. In severe cases, it may present with erythroderma, which is clinicopathologically analogous to cutaneous T‐cell lymphoma. Typically, inflammatory infiltrates in the skin lesions are mainly CD 8 + reactive T cells. However, hematologic characteristics of CAD have not been fully elucidated. Methods Twenty patients with CAD ranging in age from 45 to 86 years (median, 64), including 17 males and three females (M/F ratio, 5.7), were examined. All patients were phototested for UV light. In addition, seven of the 20 patients with extensive eruption were also tested for visible light. All biopsy specimens were obtained from the CAD eruptions ( n = 25 lesions). Histopathologic and immunohistochemical studies were performed. Furthermore, flow cytometric analysis was performed to determine the CD 4/8 ratio using peripheral blood mononuclear cells of 13 of the 20 patients. Results In 11 of the 20 patients (55%), the eruption was localized to sun‐exposed areas. Skin‐infiltrating T cells were CD 8‐dominant in the CAD eruption. Three patients (15%) showed erythroderma with a reduced CD 4/8 ratio (median, 0.7) of peripheral mononuclear cells. As for treatment, eight of the 20 patients (40%) required oral cyclosporine in addition to topical therapies. Subsequently, the reduced CD 4/8 ratio was normalized after treatment in two of the three patients with erythroderma. Conclusions We considered that there appeared to be a relationship between the reduced CD 4/8 ratio of circulating T cells (hematologic burden) and the affected area (skin burden).