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CD 8+ and FoxP3+ T‐cell infiltration in actinic cheilitis
Author(s) -
Rojas Isolde G.,
Spencer Maria L.,
Zapata Paulina A.,
Martínez Alejandra,
Alarcón Rosario,
Marchesani Francisco J.,
Tezal Mine
Publication year - 2017
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1111/ijd.13446
Subject(s) - medicine , infiltration (hvac) , dermatology , thermodynamics , physics
Background Differences in immune profile between actinic cheilitis ( AC ), a precursor of lip squamous cell carcinoma, and normal lip vermillion ( NL ) have not been elucidated. Objectives To compare density, distribution, and ratios of CD 8+ and FoxP3+ cells between AC and NL and assess their associations with clinicopathologic variables. Methods Samples of AC and NL obtained between 2001 and 2013 at the College of Dentistry of the University of Concepcion, Chile, were retrospectively analyzed for immunohistochemical detection of CD 8+ and FoxP3+ cells. Differences between groups were tested by Mann–Whitney U and Fisher's exact tests. Independent effects of cell densities and CD 8/FoxP3 ratio with AC were assessed by multiple logistic regression analysis after adjustment for potential confounding. Results A total of 62 AC and 24 NL biopsies were included. Densities of CD 8+ and FoxP3+ cells in AC were significantly higher than in NL . Conversely, the CD 8+/FoxP3+ ratio was significantly lower in AC as compared to NL . After adjustment for sun exposure, age, gender, and smoking status, a stromal FoxP3+ cell density higher than 0.35 cells/field was significantly associated with increased odds of AC (odds ratio [ OR ] = 5.01, 95% confidence interval [ CI ]: 1.18–21.31), while a stromal CD 8+/FoxP3+ ratio higher than 5.91 was associated with decreased odds of AC ( OR = 0.29, 95% CI : 0.08–1.08). Conclusions AC is characterized by increased FoxP3+ cell infiltration and a reduced CD 8/FoxP3 ratio as compared to NL . Therefore, increased infiltration of FoxP3+ cells relative to CD 8+ cells may contribute to the transition from normal to preneoplastic stages in lip carcinogenesis.