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Protective effects of tacalcitol against oxidative damage in human epidermal melanocytes
Author(s) -
Li QiLin,
Wu YanHua,
Niu Mu,
Lu XiaoJuan,
Huang YongHua,
He DanHua
Publication year - 2017
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1111/ijd.13407
Subject(s) - malondialdehyde , superoxide dismutase , reactive oxygen species , oxidative stress , medicine , hydrogen peroxide , viability assay , oxidative phosphorylation , vitiligo , staining , andrology , pathology , immunology , biochemistry , chemistry , cell
Background Oxidative damage may lead to the dysfunction of melanocytes ( MC s) and is one of the causative mechanisms involved in the pathogenesis of vitiligo. Objectives This study was designed to investigate the protective effects of the vitamin D3 analog tacalcitol on oxidative damage induced by hydrogen peroxide (H 2 O 2 ) in human epidermal MC s. Methods Human epidermal MC s were cultured and identified by l ‐ DOPA staining and HMB ‐45 immunohistochemical staining. The model of oxidative damage induced by H 2 O 2 was established, and the cells were treated with tacalcitol. The viability of MC s was determined using an MTS assay. Morphological changes in cell dendrites were observed by microscopy, and the rate of change of dendrites was calculated. The reactive oxygen species ( ROS ) level in MC s was determined using immunofluorescence microscopy. Superoxide dismutase ( SOD ) activity and malondialdehyde ( MDA ) levels in MC s were determined using the WST ‐1 and TBA methods, respectively. Results In comparison with the control group, the viability of MC s and SOD activity were significantly decreased in the H 2 O 2 group ( P < 0.05) and significantly increased in the tacalcitol group ( P < 0.05). In comparison with the control group, the rate of change of cell dendrites and levels of ROS and MDA were significantly increased in the H 2 O 2 group ( P < 0.05) and significantly decreased in the tacalcitol group ( P < 0.05). Conclusions Tacalcitol can reduce oxidative damage induced by H 2 O 2 in MC s by inhibiting intracellular ROS overproduction, increasing SOD activity, and decreasing the level of MDA , thereby reducing cell apoptosis.