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Medium‐dose ultraviolet A1 phototherapy improves SCORAD index and increases mRNA expression of interleukin‐4 without direct effect on human β defensin‐1, interleukin‐10, and interleukin‐31
Author(s) -
Bogaczewicz Jaroslaw,
Malinowska Karolina,
SysaJedrzejowska Anna,
Wozniacka Anna
Publication year - 2016
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1111/ijd.13213
Subject(s) - scorad , atopic dermatitis , interleukin , medicine , messenger rna , pathogenesis , reverse transcription polymerase chain reaction , immunology , cytokine , real time polymerase chain reaction , interleukin 4 , eczema area and severity index , biology , dermatology life quality index , gene , biochemistry , psoriasis
Abstract Background Effectiveness of ultraviolet (UV)A1 in flares of atopic dermatitis (AD) is thought to influence the expression of cytokines involved in its pathogenesis. The aim of the study was to investigate whether mRNA expression of human β defensin‐1 (hβD‐1) correlates with that of interleukin (IL)‐4, IL‐10, and IL‐31 in skin lesions in AD before and after UVA1 phototherapy, to determine whether UVA1 decreases the expression of the aforementioned mediators, and to confirm whether changes in mRNA expression correspond with the clinical efficacy of UVA1. Methods Twenty‐five patients with AD underwent medium‐dose UVA1 phototherapy. Before and after UVA1, biopsies from acute skin lesions were studied using reverse transcription and real‐time polymerase chain reaction. Results Levels of mRNA hβD‐1 correlated with those of IL‐10 and IL‐31, levels of IL‐4 mRNA correlated with those of IL‐10 and IL‐31, and IL‐10 expression correlated with that of IL‐31, both before and after UVA1. Phototherapy with UVA1 improved SCORing of Atopic Dermatitis (SCORAD) values, decreased pruritus, and increased expression of IL‐4. After UVA1, no difference was found in the mRNA expression of other molecules. The SCORAD index did not correlate with the expression of any examined mRNA either before or after UVA1. Conclusions hβD‐1, IL‐4, IL‐10, and IL‐31 are expressed in acute skin lesions in AD, and their levels correlate with each other. UVA1 improves SCORAD and pruritus and increases the expression of IL‐4 without direct effect on other molecules.