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Medium‐dose ultraviolet A1 phototherapy and mRNA expression of TSLP , TARC , IL ‐5, and IL ‐13 in acute skin lesions in atopic dermatitis
Author(s) -
Bogaczewicz Jaroslaw,
Malinowska Karolina,
SysaJedrzejowska Anna,
Wozniacka Anna
Publication year - 2016
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1111/ijd.12992
Subject(s) - thymic stromal lymphopoietin , scorad , atopic dermatitis , medicine , messenger rna , reverse transcription polymerase chain reaction , interleukin , chemokine , real time polymerase chain reaction , immunology , cytokine , chemistry , inflammation , biochemistry , psoriasis , dermatology life quality index , gene
Abstract Background The mechanisms responsible for the efficacy of ultraviolet A1 ( UVA 1) in the treatment of atopic dermatitis ( AD ) are not fully understood. Objectives This study was designed to investigate mRNA expression of thymic stromal lymphopoietin ( TSLP ), thymus‐ and activation‐regulated chemokine ( TARC ), interleukin‐5 ( IL ‐5), and IL ‐13 in AD before and after UVA 1 therapy, to determine correlations among them, and to examine whether UVA 1 influences their expression and whether it is associated with UVA 1 efficacy. Methods Twenty‐five patients with AD underwent medium‐dose UVA 1 phototherapy. Before and after UVA 1, biopsies from acute skin lesions were studied using reverse transcription and real‐time polymerase chain reaction. Results Levels of mRNA TSLP correlated with those of TARC , IL ‐5, and IL ‐13, and levels of TARC correlated with those of IL ‐5 and IL ‐13, both before and after UVA 1. Expression of IL ‐5 correlated with that of IL ‐13 only before UVA 1. SCORAD ( SCOR ing of Atopic Dermatitis) indices correlated with levels of TARC and IL ‐5 before irradiation. After UVA 1, no mRNA level correlated with the SCORAD index. Phototherapy with UVA 1 improved SCORAD values ( P < 0.001) and increased expression of TARC ( P < 0.05) but did not affect mRNA expression of TSLP , IL ‐5, or IL ‐13. Conclusions Expression levels of the mediators TSLP , TARC , IL ‐5, and IL ‐13 in AD are interrelated. Phototherapy with UVA 1 improves SCORAD indices and increases expression of TARC but has no direct effects on the expression of other molecules. It is likely that UVA 1 also interferes with or acts via intermediators on the link between IL ‐5 and IL ‐13.