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Hepatocyte growth factor‐expressing adenovirus upregulates matrix metalloproteinase‐1 expression in keloid fibroblasts
Author(s) -
Jeon Yeo Reum,
Ahn Hyo Min,
Choi Il Kyu,
Yun Chae Ok,
Rah Dong Kyun,
Lew Dae Hyun,
Lee Won Jai
Publication year - 2016
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1111/ijd.12965
Subject(s) - hepatocyte growth factor , matrix metalloproteinase , microbiology and biotechnology , extracellular matrix , multiplicity of infection , adenoviridae , reverse transcription polymerase chain reaction , messenger rna , medicine , biology , virus , immunology , genetic enhancement , gene , biochemistry , receptor
Background Keloids are marked by an overabundance of extracellular matrix. The antifibrotic effect of hepatocyte growth factor ( HGF ) is achieved by increasing the expression of matrix metalloproteinases ( MMP s) that drive extracellular matrix catabolism. As such, we cultivated an RGD ‐modified HGF ‐expressing adenovirus ( dE 1‐ RGD /lacZ/ HGF ) for introduction into keloid fibroblasts ( KF s), looking at the subsequent impact on MMP ‐1 expression. Methods KF s infected with either test virus as experimental group ( dE 1‐ RGD /lacZ/ HGF ) or its counterpart ( dE 1‐ RGD /lacZ) as control group were examined for HGF protein expression using an enzyme‐linked immunosorbent assay ( ELISA ). Collagen (types I and III ) and MMP ‐1 mRNA levels were also determined by reverse transcriptase–polymerase chain reaction, and ELISA was used to monitor MMP ‐1 protein expression. Results In KF s harboring the test virus, high levels of HGF were induced at a multiplicity of infection ratio of 50 (3260.6 ± 162.7 pg/ml) after 72 hours of incubation. Furthermore, reverse transcriptase–polymerase chain reaction and ELISA confirmed that MMP ‐1 mRNA and protein expression rose significantly in KF s after transduction by the test virus ( P < 0.05). However, mRNA levels of collagen were unaffected by the experimental group. Conclusion These results suggest that an HGF ‐expressing adenovirus may be therapeutic for keloids by increasing MMP ‐1 expression.