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The therapeutic impact of HNP ‐1 in condyloma acuminatum
Author(s) -
Zhao Shuyun,
Zhou Hong Ying,
Li Huijuan,
Yi Tao,
Zhao Xia
Publication year - 2015
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1111/ijd.12725
Subject(s) - condyloma acuminatum , transfection , recombinant dna , apoptosis , immunohistochemistry , plasmid , virology , medicine , genetic enhancement , gene , microbiology and biotechnology , biology , immunology , human papillomavirus , biochemistry
Abstract Background Condyloma acuminatum is one of the most commonly occurring sexually transmitted diseases. HNP 1 is a small antimicrobial peptide that has been reported to have antiviral activities. Aim Using the condyloma acuminatum tissue culture to resemble the situation more closely in vivo , we investigate the therapeutic effect of a recombinant plasmid encoding HNP 1 gene in condyloma acuminatum tissue. Methods Recombinant plasmid DNA carrying HNP1 c DNA was constructed and identified. Then the recombinant plasmid was transfected into a condyloma acuminatum tissue fragment, and the HNP 1 expression was determined on these tissue fragments by immunohistochemistry. TUNEL staining and flow cytometry techniques were used to examine cell apoptosis of condyloma acuminatum tissue. Relative real‐time polymerase chain reaction was used to validate antihuman papillomavirus therapeutics of the treatment groups. Results Transfected HNP 1 gene was expressed mainly in the cytoplasmic granules of the condyloma acuminatum tissues. Positive apoptotic cells were observed in condyloma acuminatum tissues transfected with the HNP 1 gene. In addition, the HPV expression was lower in the HNP 1 treatment tissues as compared to their corresponding control tissues. Conclusion The results indicate that HNP 1 can directly promote condyloma acuminatum cell apoptosis and play an antivirus role in the condyloma acuminatum tissue by limiting viral replication. These observations suggest a possible application for human HNP 1 on condyloma acuminatum therapy.