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Prevalence of human papillomavirus infection and RAS mutation in sporadic keratoacanthoma
Author(s) -
Roh Mi Ryung,
Kim Jee Hung,
Lee Sang Hee,
Oh Seung Joon,
Park Kyu Hyun,
Chung Kee Yang,
Rha Sun Young
Publication year - 2015
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1111/ijd.12669
Subject(s) - hras , neuroblastoma ras viral oncogene homolog , kras , mutation , polymerase chain reaction , papillomaviridae , hpv infection , gene , keratoacanthoma , medicine , cancer research , biology , cancer , genetics , cervical cancer , pathology , basal cell
Background RAS gene activation and its association with human papillomavirus ( HPV ) infection have been extensively studied in various cancers. However, the correlation between RAS mutations and HPV in keratoacanthoma ( KA ) has not yet been investigated. Methods Detection of HPV DNA was performed by nested polymerase chain reaction in 28 KA specimens. Molecular analysis was also performed to identify oncogenic mutations ( HRAS , KRAS , NRAS ). Statistical analyses were performed using the Fisher's exact tests. Results HPV DNA was detected in eight (28.6%) of the 28 samples, and RAS mutations were detected in eight (28.6%). Six samples had an HRAS mutation, and two showed the NRAS mutation. The presence of an RAS mutation was significantly correlated with a history of chronic sun damage ( P = 0.005). However, no significant correlation was observed between HPV infection and RAS mutation. Conclusions Our findings suggest that mutational activation of the RAS gene is a common event in KA . However, RAS oncogene activation and HPV infection seem to represent two independent factors in the development of KA .