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Allelic variation in HLA‐DRB1* loci in Syrian pemphigus vulgaris patients
Author(s) -
Harfouch Elham,
Daoud Saleh
Publication year - 2014
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1111/ijd.12184
Subject(s) - pemphigus vulgaris , medicine , allele , human leukocyte antigen , immunology , odds ratio , population , polymorphism (computer science) , genetics , antigen , biology , gene , environmental health
Background Pemphigus vulgaris ( PV ) is known to be strongly associated with particular alleles of the human leukocyte antigen ( HLA )‐ DRB 1* loci. However, to date, the association of HLA with pemphigus has not been studied in a Syrian population. Objectives We aimed to study HLA ‐ DRB 1* loci variation in Syrian PV patients. Methods We used a sequence‐specific primer polymerase chain reaction method to define the allele type of the DRB 1* loci in 91 patients with PV and 270 healthy control subjects. Results We noticed an increased frequency of HLA ‐ DRB 1*04 and DRB 1*14 alleles in patients with PV compared with healthy control subjects (67.0% vs. 26.3%; odds ratio [ OR ] = 5.7, corrected P < 0.0001 for DRB 1*04; 54.9% vs. 11.0%; OR = 9.5, corrected P < 0.0001 for DRB 1*14). Among DRB 1*04‐positive PV patients, the DRB 1*402 subtype was markedly increased compared with DRB 1*04‐positive healthy controls (84.3% vs. 26.7%; corrected P = 0.002), whereas the DRB 1*0403 variant was represented more commonly in DRB 1*04‐positive healthy individuals than in DRB 1*04‐positive PV patients (45.0% vs. 1.6%; corrected P < 0.0001). In addition, the frequency of DRB 1*11 was significantly decreased in PV patients compared with healthy controls (19.7% vs. 50.4%; OR = 0.25, corrected P < 0.0001). Conclusions The occurrence of PV is positively associated with HLA ‐ DRB 1*14 and HLA ‐ DRB 1*0402 but negatively associated with HLA ‐ DRB 1*11 in the Syrian population.