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3D mesoporous bioactive glass/silk/chitosan scaffolds and their compatibility with human adipose‐derived stromal cells
Author(s) -
Phetnin Ratiya Buapa,
Suksaweang Sag,
Giannasi Chiara,
Brini Anna Teresa,
Niada Stefania,
Srisuwan Sawitri,
Rattanachan Sirirat Tubsungnoen
Publication year - 2020
Publication title -
international journal of applied ceramic technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 57
eISSN - 1744-7402
pISSN - 1546-542X
DOI - 10.1111/ijac.13607
Subject(s) - fibroin , materials science , bioactive glass , scaffold , biocompatibility , chitosan , mesoporous material , tissue engineering , regenerative medicine , stromal cell , biomedical engineering , viability assay , nanotechnology , stem cell , silk , composite material , chemical engineering , cell , chemistry , microbiology and biotechnology , medicine , biochemistry , pathology , metallurgy , biology , engineering , catalysis
Human adipose‐derived stem/stromal cells (hASCs) have been popularly studied as cell‐based therapy in the field of regenerative medicine due to their ability to differentiate into several cell types. In this study, in order to improve the mechanical strength and bioactivity of scaffolds for bone tissue engineering, three types of mesoporous bioactive glasses with different shapes and compositions were dispersed in the silk fibroin/chitosan (SF/CS)‐based scaffolds, which were fabricated with a combination of freezing and lyophilization. The characteristic and physical properties of these composite scaffolds were evaluated. The biocompatibility was also assessed through hASCs in vitro tests. Both Alamar Blue® and Live/Dead assay® revealed that the spherical mesoporous bioactive glass doped scaffolds enhanced cell viability and proliferation. Furthermore, the addition of spherical mesoporous bioactive glass into SF/CS scaffolds encouraged hASC osteogenic differentiation as well. These results suggested that this composite scaffold can be applicable material for bone regeneration.