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Co‐expression of TLR ‐9 and MMP ‐13 is associated with the degree of tumour differentiation in prostate cancer
Author(s) -
Kalantari Elham,
Abolhasani Maryam,
Roudi Raheleh,
Farajollahi Mohammad M.,
Farhad Seif,
Madjd Zahra,
AskarianAmiri Shaghayegh,
Mohsenzadegan Monireh
Publication year - 2019
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1111/iep.12314
Subject(s) - intraepithelial neoplasia , prostate cancer , prostate , hyperplasia , matrix metalloproteinase , high grade prostatic intraepithelial neoplasia , medicine , pathology , pca3 , cancer
Summary In vitro experiments demonstrated that stimulation of Toll‐like receptor 9 ( TLR ‐9) by synthetic TLR ‐9 ligands induces the invasion of TLR ‐9‐expressing prostate cancer cells through matrix metalloproteinase 13 ( MMP ‐13). However, the clinical value of TLR ‐9 and MMP ‐13 co‐expression in the pathophysiology of the prostate is unknown. In the study, we evaluated the expression levels and clinical significance of the TLR ‐9 and MMP ‐13 in a series of prostate tissues. One hundred and eighty prostate tissues including prostate cancer ( PC a) (n = 137), high‐grade prostatic intraepithelial neoplasia ( HPIN ) (n = 18) and benign prostatic hyperplasia ( BPH ) (n = 25) were immunostained for the TLR ‐9 and MMP ‐13 markers. Subsequently, the correlation between the TLR ‐9 and MMP ‐13 staining scores and clinicopathological parameters was obtained. Higher expressions of TLR ‐9 and MMP ‐13 were found in PC a and high‐grade prostatic intraepithelial neoplasia compared to benign prostatic hyperplasia tissues. Among PC a samples, a positive relationship was revealed between the MMP ‐13 expression and Gleason score ( P < 0.001). There was a significant correlation between TLR ‐9 expression and regional lymph node involvement ( P = 0.04). The expression patterns of TLR ‐9 and MMP ‐13 markers demonstrated a reciprocal significant correlation between the two markers in the same series of prostate samples ( P < 0.001). Furthermore, the Gleason score of TLR ‐9 high / MMP ‐13 high and TLR ‐9 low / MMP ‐13 low phenotypes showed a significant difference ( P = 0.002). Higher expressions of TLR ‐9 and MMP ‐13 can confer aggressive behaviour to PC a. Therefore, these markers may be used as a valuable target for tailored therapy of PC a.