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Role of CD 97 small isoform in human cervical carcinoma
Author(s) -
He Ying,
Xu Lian,
Feng Min,
Wang Wei
Publication year - 2019
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1111/iep.12303
Subject(s) - gene isoform , gene knockdown , biology , epidermal growth factor , metastasis , cervical cancer , cancer research , cancer , cell growth , cell culture , gene , genetics
Summary Various studies revealed that elevated expression of CD 97 in carcinomas is associated with the dedifferentiation, aggressiveness and metastasis of tumour. CD97 is a protein member of the epidermal growth factor seven‐transmembrane ( EGF ‐ TM 7) family of class II TM 7 receptors. Our previous study suggested that the overexpression of CD 97 in cervical cancer was correlated with the aggressiveness of the tumour and that CD 97 might be an independent poor prognostic factor for cervical cancer patients. Based on these data, we have investigated the role of CD 97 small isoform in cervical cancer proliferation, migration and invasion in vitro. Three cervical cancer cell lines were tested and the CD 97 small isoform was found to be expressed predominantly in the SiHa cells. The mobile and invasive ability of different cervical cancer cell lines was not correlated positively with total CD 97 protein expression but was with the level of the CD 97 small isoform. Functional significance was assessed 48 hours after transient knockdown using si RNA targeting CD 97 small isoform ( CD 97/ EGF 1,2,5) or a scrambled control sequence in cervical cancer cell lines. As a result, decreased ability of migration and invasion was found in CD 97 small isoform RNA i cells, which showed, however, no change in cell proliferation. This study shed light on the role of the CD 97 small isoform in tumour progression and provides a basis for further studies to determine its function in vivo.