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Omega‐6 fatty acids down‐regulate matrix metalloproteinase expression in a coronary heart disease‐induced rat model
Author(s) -
Lu Nihong,
Du Yingrong,
Li Haiyuan,
Luo Yu,
Ouyang Bing,
Chen Yangjun,
Yang Yongrui,
Yang Lixia
Publication year - 2018
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1111/iep.12293
Subject(s) - superoxide dismutase , catalase , glutathione peroxidase , malondialdehyde , medicine , reactive oxygen species , glutathione , endocrinology , chemistry , dismutase , oxidative stress , matrix metalloproteinase , omega 3 fatty acid , antioxidant , biochemistry , fatty acid , biology , polyunsaturated fatty acid , enzyme , docosahexaenoic acid
Summary The present study investigated the therapeutic potential of omega‐6 fatty acids, according to their effects on antioxidant markers and matrix metalloproteinases ( MMP s), in coronary heart disease‐induced rats. Rats were grouped into group I (sham control), group II (control), group III (0.5 g/kg bwt of omega‐6 fatty acids) and group IV (1 g/kg bwt of omega‐6 fatty acids). Reactive oxygen species ( ROS ), malondialdehyde ( MDA ), superoxide dismutase ( SOD ), reduced glutathione ( GSH ), catalase, glutathione peroxidase (Gpx) and acetylcholinesterase ( AC hE) enzyme activities were determined. ROS and MDA were substantially reduced, whereas SOD , catalase, Gpx and AC hE were significantly increased, following supplementation with omega‐6 fatty acids. MMP ‐2 mRNA expression was drastically increased by 95% in group II . Treatment significantly reduced MMP ‐2 mRNA expression by 12.3% and 26.7% in groups III and IV respectively. MMP ‐9 mRNA expression drastically increased, by 121%, in group II . Treatment significantly reduced MMP ‐9 mRNA expression by 22.6% and 29.4% in groups III and IV respectively. MMP ‐2 protein expression was drastically increased, by 81%, in group II . Treatment significantly reduced MMP ‐2 protein expression by 9.4% and 26% in groups III and IV respectively. MMP ‐9 protein expression was drastically increased, by 100%, in group II . Treatment significantly reduced MMP ‐9 protein expression by 18.9% and 26.9% in groups III and IV respectively. In summary, the consumption of omega‐6 fatty acids significantly decreased MDA and ROS , while SOD , catalase, GHS , Gpx and AC hE were increased. Furthermore, omega‐6 fatty acids significantly downregulated MMP ‐2 and MMP ‐9 expression in our coronary heart disease‐induced rat model.