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Carnosine decreased oxidation and glycation products in serum and liver of high‐fat diet and low‐dose streptozotocin‐induced diabetic rats
Author(s) -
Aydın Abdurrahman Fatih,
Bingül İlknur,
Küçükgergin Canan,
DoğanEkici Işın,
Doğru Abbasoğlu Semra,
Uysal Müjdat
Publication year - 2017
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1111/iep.12252
Subject(s) - glycation , medicine , endocrinology , streptozotocin , malondialdehyde , chemistry , oxidative stress , diabetes mellitus , triglyceride , steatosis , cholesterol
Summary High‐fat diet ( HFD ) and low‐dose streptozotocin ( STZ )‐treated rats provide useful animal model for type II diabetes mellitus. Oxidative stress and advanced glycation end products ( AGE s) play a role in the development of diabetic complications. Carnosine ( CAR ) has anti‐oxidant and anti‐glycating properties. We investigated the effects of CAR on oxidation and glycation products in HFD + STZ rats. Rats were fed with HFD (60% of total calories from fat) for 4 weeks, and then a single dose of STZ (40 mg/kg; i.p.) was applied. Rats with blood glucose levels above 200 mg/ dl were fed with HFD until the end of the 12th week. CAR (250 mg/kg body weight; i.p.; five times a week) was administered to the rats for the last four weeks. CAR significantly decreased serum triglyceride ( TG ) (57.7%), cholesterol (35.6%) levels and hepatic marker enzyme activities of HFD + STZ rats. It significantly reduced serum reactive oxygen species ( ROS ) (23.7%), AGE s (13.4%) and advanced oxidized protein products ( AOPP ) (35.9%) and hepatic TG (59%), ROS (26%), malondialdehyde ( MDA ) (11.5%), protein carbonyl ( PC ) (19.2%) and AGE (20.2%) levels. Liver steatosis and hepatocyte ballooning were also significantly reduced. However, CAR treatment did not alter serum glucose and blood glycated haemoglobin and hepatic anti‐oxidant enzyme activities/ mRNA expressions in HFD + STZ rats. Our results indicate that CAR decreased accumulation of oxidation and glycation products, such as MDA , AGE , AOPP and PC in the serum and liver and ameliorated hepatic dysfunction in HFD + STZ rats. This effect may be related to its anti‐oxidative, anti‐glycating, and anti‐lipogenic potential.