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Functional roles of CSPG 4/ NG 2 in chondrosarcoma
Author(s) -
Jamil Nuor S. M.,
Azfer Asim,
Worrell Harrison,
Salter Donald M.
Publication year - 2016
Publication title -
international journal of experimental pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.671
H-Index - 72
eISSN - 1365-2613
pISSN - 0959-9673
DOI - 10.1111/iep.12189
Subject(s) - chondrosarcoma , aggrecanase , cancer research , biology , chemistry , microbiology and biotechnology , medicine , pathology , osteoarthritis , alternative medicine , articular cartilage
Summary CSPG 4/ NG 2 is a multifunctional transmembrane protein with limited distribution in adult tissues including articular cartilage. The purpose of this study was to investigate the possible roles of CSPG 4/ NG 2 in chondrosarcomas and to establish whether this molecule may have potential for targeted therapy. Stable knock‐down of CSPG 4/ NG 2 in the JJ 012 chondrosarcoma cell line by sh RNA resulted in decreased cell proliferation and migration as well as a decrease in gene expression of the MMP (matrix metalloproteinase) 3 protease and ADAMTS 4 (aggrecanase). Chondrosarcoma cells in which CSPG 4/ NG 2 was knocked down were more sensitive to doxorubicin than wild‐type cells. The results indicate that CSPG 4/ NG 2 has roles in regulating chondrosarcoma cell function in relation to growth, spread and resistance to chemotherapy and that anti‐ CSPG 4/ NG 2 therapies may have potential in the treatment of surgically unresectable chondrosarcoma.